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TOYOPEARL AF-Tresyl-650M

TOYOPEARL AF-Tresyl-650M is an activated resin that is highly reactive toward amine and thiol groups. It is provided in dry form, ready for reaction in buffered solutions containing the ligand to be coupled. Coupling is accomplished in a neutral to slightly alkaline (pH 7 - 8) solution. Under such conditions even proteins of limited stability may be successfully coupled. Coupling leads to the formation of a highly stable secondary amine or thio-ether linkage.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
14471TOYOPEARL AF-Tresyl-650M5 g65  303
14472TOYOPEARL AF-Tresyl-650M100 g65  3,561
14905TOYOPEARL AF-Tresyl-650M200 g65  6,524
14906TOYOPEARL AF-Tresyl-650M1 kg65  -
18371TOYOPEARL AF-Tresyl-650M5 kg65  -
          

Product Attributes

Pore size (mean): 100 nm
Particle size (mean): 65 µm
Pressure rating: 0.3 MPa
Shipping condition: dry
Storage temperature: 4 °C


Structure:

AFC_AF-Tresyl-650M_Structure.png

 
 
 
 
    

Purification of Ferredoxin-NADP Reductase


AFC_AF-Tresyl-650_Fig12_rev.png


Resin: Synechococcus Fd-TOYOPEARL
Column size: 22 mm ID × 10 cm
Mobile phase: 1. Load with crude spinach FNR in
20 mmol/L Tris-HCl, pH 7.5
2. Wash with 20 mmol/L Tris-HCl,
pH 7.5
3. Elute with 20 mmol/L Tris-HCl, pH 7.5 with 0.25 mol/L NaCl
Flow rate: 16 cm/hr (1 mL/min)
Detection: UV @ 275 nm, specific activity
Sample: spinach FNR

For additional applications, please see our applications database.

 
        

Coupling procedure


AFC_AF-Tresyl_coupling.png

 

Representative coupling densities

Protein coupled g/L resin
soybean trypsin inhibitor 16
protein A 1.9
concanavalin A 13
α1-antitrypsin 12.3
α-chymotrypsin 12.5
myoglobin 12.4
bovine serum albumin 12.4
human IgG 10.0
lysozyme 60

coupling agent: not required
optimal pH: 7.0 - 9.0

 
Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM13 Instruction Manual -- TOYOPEARL AF-Tresyl-650MInstruction Manual
PCAT04 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- AffinityProduct Catalog
RIS0007 TOYOPEARL AF-Tresyl-650MResin Information Sheet
P/NDescriptionPrice ($) 
43400TOYOPEARL AFFIPAK ACT326