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TOYOPEARL AF-rProtein L-650F

TOYOPEARL AF-rProtein L-650F is a protein L resin that exhibits the highest binding capacity for antibody fragments that lack a Fc-portion (e.g. Fab, scFv, Dab, etc.).

TOYOPEARL AF-rProtein L-650F resin does not only offer the highest binding capacity, but also has the ability to bind to antibody classes that do not have the affinity to Protein A, such as IgM and IgA. 

The selected recombinant protein L ligand used in the TOYOPEARL AF-rProtein L-650F resin is alkali-stable and is therefore able to withstand the high alkaline conditions necessary for clean-in-place (CIP). This resin exhibits stability in various solutions and over a wide pH range, as well as demonstrates excellent pressure-flow rate properties and will withstand back pressures up to 0.2 MPa.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
23486TOYOPEARL AF-rProtein L-650F10 mL45  799
23487TOYOPEARL AF-rProtein L-650F25 mL45  1,377
23488TOYOPEARL AF-rProtein L-650F100 mL45  2,754
23489TOYOPEARL AF-rProtein L-650F1 L45  -
23490TOYOPEARL AF-rProtein L-650F5 L45  -
       

Product Attributes

Pore size (mean): 100 nm
Particle size (mean): 45 µm
Pressure rating: 0.2 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life (estimated): 10 years

Structure:

AFC_rProtein_L-650F_structure.jpg

 
 
           

Separation of Fab and Fc (papain digestion)

ProL_AF-rProtein-L-650F-Slide11.png


Resin: TOYOPEARL AF-rProtein L-650F
Column size: 5 mm ID × 2.5 cm
Mobile phase: Buffer A: 0.1 mol/L Na-Citrate, pH 6.5
Buffer B: 0.1 mol/L Citrate, pH 2.05
Gradient: B = 0% (0 to 1 min)
B = 0% to 100% (1 min to 16 min)
Flow rate: 0.5 mL/min (residence time = 1 min)
Detection: UV @ 280 nm
Injection: 3 mg BI_mAb2 papain digestion

Purification of scFv (mammalian cell culture)

ProL_AF-rProtein-L-650F-Slide13.png


Resin: TOYOPEARL AF-rProtein L-650F
Column size: 5 mm ID × 3.5 cm (0.5 mL)
Equib. & flash wash: 0.1 mol/L Na-Citrate, pH 6.5
Intermediate wash: 0.1 mol/L Na-Citrate, pH 6.5 +
1 mol/L Arg-HCl, 10% iPrOH
Elute: 0.1 mol/L Citrate, pH 2.3
CIP: 0.05 mol/L NaOH
Flow rate: 0.35 mL/min (residence time = 1.4 min)
Detection: UV @ 280 nm
Sample: hu-scFv1 CHO culture sup. 40 mL

For additional applications, please see our applications database.

 
      

Highest Dynamic Binding Capacity for Fab

ProL_AF-rProtein-L-650F-PD1.png

Excellent Stability in 0.1 mol/L NaOH

ProL_AF-rProtein-L-650F-PD2.png

Widest Affinity for Fab Classes

Species Class Affinity
General Kappa light chain ++

Lambda light chain -

Heavy chain -

Fab ++

ScFv ++

Dab ++
Human IgG(1-4) +

IgA +

IgD +

IgE +

IgM +
Mouse IgG1 +
IgG2a +
IgG2b +
IgA +

IgM +
Rat IgG1 +

IgG2a,b,c +
IgA +
Chicken IgM +
IgY +
Code #DescriptionLiterature Type
AN100 Purification of Fab and scFv using High Binding Capacity TOYOPEARL AF-rProtein L-650FApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PO46 TOYOPEARL AF-rProtein L-650FProduct Overview
RDS5060 TOYOPEARL AF-rProtein L-650F ResinOperating Conditions and Specifications
P/NDescriptionPrice ($) 
45162MiniChrom TOYOPEARL AF-rProtein L-650F728
OC41MDAFPL-650FResin Seeker TOYOPEARL AF-rProtein L-650F 96-well plate kit263
23494ToyoScreen AF-rProtein L-650F, 1 mL690
23495ToyoScreen AF-rProtein L-650F, 5 mL (qty. 1)637
23496ToyoScreen AF-rProtein L-650F, 5 mL (qty. 5)2,070
45065ToyoScreen RoboColumns AF-rProtein L-650F, 200 µL259
45066ToyoScreen RoboColumns AF-rProtein L-650F, 600 µL900