Process Media

TOYOPEARL AF-rProtein L-650F

TOYOPEARL AF-rProtein L-650F is a protein L resin that exhibits the highest binding capacity for antibody fragments that lack a Fc-portion (e.g. Fab, scFv, Dab, etc.).

TOYOPEARL AF-rProtein L-650F resin does not only offer the highest binding capacity, but also has the ability to bind to antibody classes that do not have the affinity to Protein A, such as IgM and IgA. 

The selected recombinant protein L ligand used in the TOYOPEARL AF-rProtein L-650F resin is alkali-stable and is therefore able to withstand the high alkaline conditions necessary for clean-in-place (CIP). This resin exhibits stability in various solutions and over a wide pH range, as well as demonstrates excellent pressure-flow rate properties and will withstand back pressures up to 0.2 MPa.


P/NDescriptionResin VolumeParticle Size (µm)   Price
23486TOYOPEARL AF-rProtein L-650F10 mL45  558
23487TOYOPEARL AF-rProtein L-650F25 mL45  964
23488TOYOPEARL AF-rProtein L-650F100 mL45  1,927
23489TOYOPEARL AF-rProtein L-650F1 L45  -
23490TOYOPEARL AF-rProtein L-650F5 L45  -

Product Attributes

Pore size (mean): 100 nm
Particle size (mean): 45 µm
Pressure rating: 0.2 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life (estimated): 10 years




Separation of Fab and Fc (papain digestion)


Resin: TOYOPEARL AF-rProtein L-650F
Column size: 5 mm ID × 2.5 cm
Mobile phase: Buffer A: 0.1 mol/L Na-Citrate, pH 6.5
Buffer B: 0.1 mol/L Citrate, pH 2.05
Gradient: B = 0% (0 to 1 min)
B = 0% to 100% (1 min to 16 min)
Flow rate: 0.5 mL/min (residence time = 1 min)
Detection: UV @ 280 nm
Injection: 3 mg BI_mAb2 papain digestion

Purification of scFv (mammalian cell culture)


Resin: TOYOPEARL AF-rProtein L-650F
Column size: 5 mm ID × 3.5 cm (0.5 mL)
Equib. & flash wash: 0.1 mol/L Na-Citrate, pH 6.5
Intermediate wash: 0.1 mol/L Na-Citrate, pH 6.5 +
1 mol/L Arg-HCl, 10% iPrOH
Elute: 0.1 mol/L Citrate, pH 2.3
CIP: 0.05 mol/L NaOH
Flow rate: 0.35 mL/min (residence time = 1.4 min)
Detection: UV @ 280 nm
Sample: hu-scFv1 CHO culture sup. 40 mL

For additional applications, please see our applications database.


Highest Dynamic Binding Capacity for Fab


Excellent Stability in 0.1 mol/L NaOH


Widest Affinity for Fab Classes

Species Class Affinity
General Kappa light chain ++

Lambda light chain -

Heavy chain -

Fab ++

ScFv ++

Dab ++
Human IgG(1-4) +

IgA +

IgD +

IgE +

IgM +
Mouse IgG1 +
IgG2a +
IgG2b +
IgA +

IgM +
Rat IgG1 +

IgG2a,b,c +
IgA +
Chicken IgM +
IgY +
Code #DescriptionLiterature Type
AN100 Purification of Fab and scFv using High Binding Capacity TOYOPEARL AF-rProtein L-650FApplication Note
AN103 Capture of a scFv from E. coli using TOYOPEARL AF-rProtein L-650FApplication Note
BR10 Best-In-Class Bioprocessing ResinsBrochure
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT04 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- AffinityProduct Catalog
PO46 TOYOPEARL AF-rProtein L-650FProduct Overview
RIS0010 TOYOPEARL AF-Protein-L-650FResin Information Sheet
P/NDescriptionPrice ($) 
OC41MDAFPL-650FResin Seeker TOYOPEARL AF-rProtein L-650F 96-well plate kit286
45228SkillPak Antibody 1 mL column library442
45232SkillPak Best in Class 1 mL column library 391
45266SkillPak Best in Class 5 mL column library1,330
45200SkillPak TOYOPEARL AF-rProtein L-650F 1 mL column117
45221SkillPak TOYOPEARL AF-rProtein L-650F 1 mL columns (qty. 5)576
45257SkillPak TOYOPEARL AF-rProtein L-650F 5 mL column339
23494ToyoScreen AF-rProtein L-650F, 1 mL (qty. 5)690
23495ToyoScreen AF-rProtein L-650F, 5 mL (qty. 1)637
23496ToyoScreen AF-rProtein L-650F, 5 mL (qty. 5)2,070
45065ToyoScreen RoboColumns AF-rProtein L-650F, 200 µL354
45066ToyoScreen RoboColumns AF-rProtein L-650F, 600 µL1,228