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LenS3 Multi-Angle Light Scattering Detector

LENS3

The LenS3 MALS detector offers a revolutionary approach for the measurement of molecular weight (MW) and radius of gyration (Rg) of synthetic polymers, polysaccharides, proteins, and biopolymers. A novel optical design, a unique cell-block assembly, and a new calculation methodology make the LenS3 MALS detector the first groundbreaking innovation in the light scattering technology arena in decades!

Featuring a patent pending cell geometry and a lower wavelength laser (at 505 nm), the LenS3 MALS detector allows direct measurement of molecular weight and provides best-in-class sensitivity. In addition, with an improved normalization process and a new method for utilizing the angular dependence, the determination of radius of gyration of smaller macromolecules below 10 nm in gyration size, Rg, is now possible for the first time in history!

The LenS3 MALS detector is driven by SECview™ software, capable of GPC system/hardware control, multi-channel data acquisition, data processing and analysis. Totally focused around enhancing the user-interface experience, SECview is a welcoming fresh perspective that streamlines the complex calculations required by the advanced detectors and is the only GPC/SEC data processing module today that performs analysis of sample-of-interest using multiple calibration methods simultaneously.
 
P/NDescriptionPrice
($)		 
0040000LenS3 Multi-Angle Light Scattering Detector-


Specifications

Light scattering
Number of measurement angles 3
Position of the measurement angles LALS (10°)   RALS (90°)   HALS (170°)
Cell geometry proprietary conical flow path (single inlet, dual outlets)
Laser source type diode
Laser power 20 mW +/- 5
Laser wavelength 505 nm
Laser temperature control yes
Wetted material teflon, PEEK, glass, stainless steel
Maximum flow rate 2 mL/min
Inlet position front or side
Baseline noise (in THF @ 1 mL/min) < 1 mV
Baseline drift (in THF @ 1 mL/min) < 1 mV / 30 min
MW range < 200 to 107 Da
Rg range < 2 nm to > 50 nm (in progress)



General
A/D board channels / resolution 8 channels / 24 bits
Acquistion rate 10 Hz
Dynamic range +/- 10 V
Analog inputs RI, UV and start signal
Connection to PC ethernet
Dimensions 36.5 (W) × 48.5 (D) × 13 (H) mm = 14.4" × 19.1" × 5.1"
Weight 16 kg = 35 lbs
Intellectual property PCT/US19/12090: Light Scattering Detectors and Sample Cells for the Same
PCT/US19/12095: Light Scattering Detectors and Methods for the Same


Radius of Gyration Below 10 nm

System & Columns

  • EcoSEC GPC System with RI detector
  • LenS3 Multi-Angle Light Scattering detector
  • 1 × TSKgel GMHHR-H (7.8 mm ID × 30 cm) column

Samples & Conditions

  • Polystyrene (PS) standards below 100K
  • THF at 1.0 mL/min; T = 35 °C (pumps, column
    and RI)
  • Concentration = 1 - 3 mg/mL; injection volume = 50 µL

LALS (red), RALS (blue) and HALS (green) signals with baselines and integration limits for PS 40K standard

LenS_PD_fig1A-rev.png

Measured values of radius of gyration, Rg,z, of PS standards

Sample Mw Rg,z Literature values*
PS100K 100,400 12.0 13.2
PS40K 40,800 7.6 8.1
PS30K 30,100 6.6 6.8
PS20K 18,600 5.5 5.3
PS10K 10,700 4.6 3.9

* Literature values calculated using the correlation
  Rg = 0.0245•MW0.546

Angular dissymetry plot for PS 30K

LenS_PD_fig1B-a.png

LenS_PD_fig1b-b.png


Superior Sensitivity

System & Columns

  • EcoSEC GPC System with RI detector
  • LenS3 Multi-Angle Light Scattering detector
  • 1 × TSKgel GMHHR-H (7.8 mm ID × 30 cm) column

Samples & Conditions

  • Tosoh 100K polystyrene (PS) standard F-10
  • THF at 1.0 mL/min; T = 35 °C (pumps, column
    and RI)
  • Decreasing injected mass

LALS signals for decreasing injected mass of PS 100K standard F-10

LALS signals for decreasing injected mass of PS 100K standard F-10

Molecular weights by LALS and RALS for decreasing injected mass of PS 100K standard F-10

Injected mass MW by LALS MW by RALS
0.7 µg 102,110 101,570
70 ng 101,520 100,960
35 ng 101,150 100,870
15 ng – inj. 1 - 102,180
15 ng – inj. 2 - 100,980
15 ng – inj. 3 - 101,390
15 ng – inj. 4 - 99,800
15 ng – inj. 5 - 100,760
15 ng – Average - 101,020 ± 780 (0.77%)

RALS signals for decreasing injected mass of PS 100K standard F-10

RALS signals for decreasing injected mass of PS 100K standard F-10

Code #DescriptionLiterature Type
AN115 Analysis of Oligonucleotides by SECApplication Note
AN119 Characterization of Recombinant Protein Biotherapeutic using TSKgel UP-SW2000 Size Exclusion Chromatography Column In-Line with LenS3 MALS DetectorApplication Note
AN125 Molecular Weight Determination of VLPs Using LenS3 Multi-Angle Light Scattering DetectorApplication Note
AN126 Characterization of Biomacromolecules by FPLC coupled with the LenS3 MALS DetectorApplication Note
AN129 SEC-MALS of Antibody Therapeutics -- A Robust Method for In-Depth Sample CharacterizationApplication Note
AN133 Monitoring Structural Changes in Polysaccharides using SEC-MALSApplication Note
AN137 Trimeric SARS-COV-2 Spike Protein Analysis with SEC Coupled with MALSApplication Note
AN138 SEC-MALS Sensitively Quantifies Adeno-Associated Viruses and Detects ImpuritiesApplication Note
AN139 Analysis of PEGylated Antibody Fragments using SEC-MALSApplication Note
AN141 SEC-MALS Analysis Determines Molecular Weight and Oligomeric State of Membrane ProteinsApplication Note
BR11 A New Paradigm in Light Scattering Technology: Solution for advanced GPC/SEC detectionBrochure
PD21 Increased Sensitivity with LenS3 MALS DetectorPerformance Data
TN001 Expanding the Boundaries of Light Scattering DetectionTechnical Note
TP229 Two-Step Purification Process for Mouse Monoclonal Antibody from Hybridoma Cell LineTechnical Presentation
WP001 Light Scattering for Determination of Molecular Weight and Radius of GyrationWhite Paper
P/NDescriptionPrice ($) 
0040001UHPLC Conversion Service Kit for LenS3 MALS Detector-