SkillPak TOYOPEARL HW-40F 1 mL columns (qty. 5)

SkillPak TOYOPEARL HW-40F 1 mL columns (qty. 5) are designed for fast method development, resin screening or sample concentration of biomolecules.

SkillPak 1 mL pre-packed columns are designed for your purification and separation tasks from platform design to pilot scale. These columns are ready to use upon receipt and show excellent physical strength and ideal flow characteristics for industrial downstream processing.

SkillPak 1 mL columns guarantee optimal performance and can be operated with commonly used low or medium pressure liquid chromatography systems. They are reproducibly packed and take into account the varying compressibility of each resin. This provides an accurate representation of conditions found in full scale columns.

P/N	Description	Resin Volume	Particle Size (µm)			Price ($)
0045223	SkillPak TOYOPEARL HW-40F 1 mL columns (qty. 5)	5 mL	45			281	Inquire

Resin Attributes

Pore size (mean):	5 nm
Exclusion limit:	10,000 Da
Particle size (mean):	45 µm
Pressure rating:	0.3 MPa
Shipping buffer:	20% ethanol
pH stability:	2-13
Shelf life:	10 years

Please see the SkillPak 1 mL instruction manual for column specifications.

Molar Mass Separation Ranges

Polyethylene glycols and oxides	100 - 3,000 Da
Dextrans	100 - 7,000 Da
Globular proteins	100 - 1 × 10⁴Da

Structure:

Calibration Curves for Globular Proteins

Resins:	A. TOYOPEARL HW-40 B. TOYOPEARL HW-50 C. TOYOPEARL HW-55 D. TOYOPEARL HW-65 E. TOYOPEARL HW-75
Column size:	22 mm ID × 30 cm
Mobile phase:	0.06 mol/L phosphate buffer, pH 7.0, + 0.06 mol/L KCl
Detection:	UV @ 280 nm
Temperature:	ambient
Samples:	protein standards
Legend:	V_e= elution volume V_c= column volume

Code #	Description	Literature Type
BR12	SkillPak Pre-packed Columns	Brochure
IM19	Instruction Manual -- SkillPak 1 mL Columns	Instruction Manual

Process Development

SkillPak TOYOPEARL HW-40F 1 mL columns (qty. 5)

Resin Attributes

Molar Mass Separation Ranges

Calibration Curves for Globular Proteins