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HPLC Columns - Affinity FAQ's

The following are Affinity Chromatography HPLC column FAQ's. Please click on the corresponding tab below for the questions and answers.

  1. What is the support material for Tosoh Bioscience affinity resins?

  2. What are the ligand concentrations, adsorption capacities and test analytes for the various Affinity column supplied by Tosoh Bioscience?

  3. Can I get a TSKgel Affinity LC column with non-metal wetted parts?

  4. How do I load and desorb protein from a TSKgel affinity column?

  5. What endfitting do I use with my particular TSKgel Affinity column? 

 

1. What is the support material for Tosoh Bioscience affinity resins?

TSKgel affinity chromatography columns are based upon the G5000PW methacrylate porous resin. This “5PW” media is hydrophilic, with 100 nm pores and an estimated protein size exclusion limit of 1000 kDa.

 

2. What are the ligand concentrations, adsorption capacities and test analytes for the various Affinity column supplied by Tosoh Bioscience?

Characteristics of TSKgel AFC columns:

Column packing

Ligand type

Ligand concentration

Adsorption capacity

Sample

Boronate-5PW m-aminophenyl boronate not available 40 µmol/mL - resin sorbitol
Chelate-5PW iminodiacetic acid 20 µmol/mL - resin
not available not available
Tresyl-5PW
tresyl
ca. 20 µmol/mL - resin
>60 mg/g dry resin (coupling capacity)
soybean trypsin inhibitor

 

3. Can I get a TSKgel Affinity LC column with non-metal wetted parts? 

Yes. We supply glass columns in two formats: 5 mm ID × 5 cm and 8 mm ID × 7.5 cm with 10 µm pore size Pyrex frits.

Shipping solvents for TSKgel AFC columns:

Column

Shipping solvent

Boronate-5PW distilled water
Chelate-5PW acetate buffer
Tresyl-5PW distilled water

 

4. How do I load and desorb protein from a TSKgel affinity column?

Specific instructions for retaining your protein as well as desorption procedures are included with each affinity column. Keep in the mind the analyte requires some structural feature that coincides with the retention mechanism of the affinity resin.

 

5. What endfitting do I use with my particular TSKgel Affinity column?

Please visit the HPLC Columns Accessories section of the product catalog for the available fittings to be used with your TSKgel column.  Question #10 within the General FAQ category includes additional information on fittings and connectors.