1. What does “HW” stand for when talking about TOYOPEARL® HW size exclusion resin?
Literally, the HW stands for Hydrophilic Water-compatible; referring to the nature of the polymeric bead used for the size exclusion resin. The size exclusion bead is also the backbone all of the other TOYOPEARL® resins.
2. How are the functional groups attached to the base beads?
All functional groups are attached to the base bead through an ether linkage to a proprietary R group extending from the base bead.
3. What is the stability data for TOYOPEARL® resins?
Stability data is collected for 10 years from the date of production. TOYOPEARL® resins have a proven stability of 10 years (except for those resins less than 10 years old!) without any adverse effects on performance.
4. What is the stability data for TSKgel® PW resins?
The TSKgel® PW resins have the same polymer chemistry as TOYOPEARL® resins and also the same post polymerization functionalization chemistry. The only difference is that the degree of crosslinking is higher which helps them withstand the intrisically higher back pressures generated by their smaller particle sizes. For TSKgel® PW resins , stability data is also collected for 10 years from the date of production. TSKgel® PW resins have a proven stability of 10 years (except for those resins less than 10 years old!) without any adverse effects on performance.
5. Does Tosoh Bioscience offer any Reversed Phase resins in bulk?
Tosoh Bioscience does not carry Reverse Phase Chromatography (RPC) resins in bulk. We do offer a variety of Hydrophobic Interaction Chromatography (HIC) resins as an alternative to Reversed Phase resins which do not require the use of hazardous organic solvents.
6. How do I assemble a ToyoScreen® without trapping air?
For step-by-step instructions, watch our instructional video or refer to the ToyoScreen® Instruction Manual.
7. How do I go about placing an order for 5 or more liters of resins?
Contact our customer service department for a quote. Lead time on shipment of an order is dependent on the quantity ordered and availability. Tosoh Bioscience chromatography media is manufactured in Japan and inventory is stocked in our Ohio facility for shipment within the U.S. Large volume orders of resin may require direct shipment from Japan and may take several weeks if the quantity requested is available.
8. What is the best method for packing TOYOPEARL® resin?
TOYOPEARL® media is rated at a maximum pressure limit of 3 bar/ 45psi. This is the recommended pressure for achieving an optimally packed chromatography bed, however this may be limited by pressure constraints by instrumentation and equipment. TOYOPEARL® resin should be packed in high salt buffer at a slurry concentration no greater than 60% to achieve maximum bed compression. Packing in salt results in a more consistent pressure drop across the column relative to packing in water at the same flow rate. After the bed height is stable under pressure, mark the bed height and lower the top adapter 0.2 - 0.5 cm into the bed to prevent a void from developing during normal chromatography operation. For additional information about column packing with our resins, please see our instructional videos.
9. Where can I get a current SDS for TOYOPEARL® and TSKgel® resins?
You can download it as a pdf file by visiting our SDS page, or you can contact our customer service department at (866) 527-3587 to receive a copy.