Solutions

Best-in-Class Retention Time Precision

Molar mass averages can be affected by changes in the environment and measuring conditions. Generally, these variations are the result of one or more factors including flow rate reproducibility, baseline drift and injection reproducibility. In addition to controlling column temperature, Tosoh engineers added temperature control for both pumps, the degasser chamber and inlet and outlet tubing on the EcoSEC Elite GPC System to deliver top GPC analysis performance.

Figure 1 and Table 1 demonstrate the superiority of the EcoSEC Elite GPC System for the determination of weight-average molar masses.


Vinyl chloride/vinyl acetate copolymer
Measurement date Mw CV(%)
Day 1 23165 0.305
2 23205 0.216
3 23029 0.109
4 23199 0.215
5 23091 0.182
6 23005 0.167
AVG. 23116 0.199
RSD 86.862 -
CV(%) 0.376 -
Reproducibility-Mw_EcoSEC Elite

  Intra day (n=10) CV: 0.199%
  Inter day (n=6) CV: 0.376%

Sample column:   
Reference column:
Mobile phase:   
Flow rate:   
Detection:  
Temperature:   
Injection vol.:   

TSKgel SuperMultiporeHZ-M × 2
TSKgel SuperH-RC
THF
0.35 mL/min, Sample and Reference
RI (EcoSEC Elite GPC System)
40 °C
10 µL (1.0 g/L)

Figure 2 and Table 2 demonstrate the superiority of the EcoSEC Elite GPC System for elution time reproducibility.


F-2 F-10
Measurement
date
Retention
time (min)
CV
(%)
Retention
time (min)
CV
(%)
Day 1 8.048 0.014 6.887 0.011
2 8.051 0.013 6.889 0.010
3 8.051 0.012 6.889 0.014
4 8.049 0.016 6.887 0.010
5 8.053 0.023 6.890 0.019
6 8.051 0.016 6.888 0.016
AVG. 8.050 0.016 6.888 0.013
RSD 0.002 - 0.001 -
CV(%) 0.022 - 0.018 -
Reproducibility-Elution_EcoSEC Elite

  Intra day (n=10) F2 CV: 0.016%; F10 CV: 0.013%
  Inter day (n=6) F2 CV: 0.022%; F10 CV: 0.018%

Sample column:   
Reference column:
Mobile phase:   
Flow rate:   
Detection:  
Temperature:   
Injection vol.:   

TSKgel SuperMultiporeHZ-M × 2
TSKgel SuperH-RC
THF
0.35 mL/min, Sample and Reference
RI (EcoSEC Elite GPC System)
40 °C
10 µL (0.2 g/L)