Solutions

TSkgel BioAssist DS Columns

TSKgel BioAssist DS Desalting Columns

TSKgel BioAssist DS columns are designed for the desalting and buffer exchange of proteins and polynucleotides at semi-preparative scale. Packed with 15 μm polyacrylamide beads in PEEK hardware, TSKgel BioAssist DS columns show excellent desalting performance.

The novel* hydrophilic highly cross-linked polyacrylamide beads exhibit superior mechanical strength compared with conventional hydrophilic polyacrylamide beads and cross-linked dextran beads. This increase in strength is what allows the use of the small spherical 15 μm beads. TSKgel BioAssist DS columns can be operated in standard HPLC systems to quickly and efficiently reduce salt and/or buffer concentrations of collected protein or nucleic acid fractions.

*US patent number 7,659,348 

Calibration Curve

The figure shows the calibration curve of a TSKgel BioAssist DS column for polyethylene glycol standards.

BioAssistDS_Cal-curves.png
Column:

Mobile phase:
Flow rate:
Samples:
Custom TSKgel BioAssist DS column,
6 mm ID × 15 cm
distilled H2
0.5 mL/min
ethylene glycol, PEGs

 


Base Material: Urea cross-linked 
polyacrylamide
 
Particle Size (mean): 15 µm  
Pore Size (mean): Excludes 2500 Da PEG                     Visit the TSKgel BioAssist DS product page.
Particle Porosity: ca. 60%                  
Mechanical Strength: < 4 MPa