TSKgel® BioAssist DS Desalting Columns
TSKgel® BioAssist DS columns are designed for the desalting and buffer exchange of proteins and polynucleotides at semi-preparative scale. Packed with 15 μm polyacrylamide beads in PEEK hardware, TSKgel® BioAssist DS columns show excellent desalting performance.
The novel* hydrophilic highly cross-linked polyacrylamide beads exhibit superior mechanical strength compared with conventional hydrophilic polyacrylamide beads and cross-linked dextran beads. This increase in strength is what allows the use of the small spherical 15 μm beads. TSKgel® BioAssist DS columns can be operated in standard HPLC systems to quickly and efficiently reduce salt and/or buffer concentrations of collected protein or nucleic acid fractions.
*US patent number 7,659,348
Calibration Curve
The figure shows the calibration curve of a TSKgel® BioAssist DS column for polyethylene glycol standards.
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Column:
Mobile phase:
Flow rate:
Samples: |
Custom TSKgel® BioAssist DS column,
6 mm ID × 15 cm
distilled H20
0.5 mL/min
ethylene glycol, PEGs |
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