Solutions

TSKgel SuperSW

TSKgel SuperSW Columns for Higher Sensitivity Protein Analysis


TSKgel SuperSW columns, introduced in 1997, have become synonymous with analyzing protein molar masses in the emerging field of biotechnology.

TSKgel SuperSW columns contain smaller particles than TSKgel SWXL columns; 4 micron versus 5 micron. In addition, the column internal diameter has been reduced from 7.8 mm ID to 4.6 mm ID to provide higher sensitivity in sample-limited cases and to cut down on solvent use.

HPLC System Requirements

It is important to employ an HPLC system that is optimized with regards to extra-column bandbroadening to take full advantage of the high column efficiency that can be obtained on TSKgel SuperSW columns. See Separation Report 95 for recommendations.

Calibration Curves

The figures show the calibration curves for globular proteins (A) and for polyethylene oxides and glycols (B) for TSKgel Super SW columns.

 SuperSW_Cal-Curve1.png

 SuperSW_Cal-Curve2.png
Columns:

Mobile phase:
Flow rate:
Detection:
Samples:
TSKgel SuperSW columns,
4.6 mm ID × 30 cm
0.2 mol/L phosphate buffer, pH 6.7
0.35 mL/min
UV @ 280 nm
standard proteins (5 µL, 0.1 g/L each)
1. thyroglobulin
2. g-globulin       
3. bovine serum albumin    
4. β-lactoglobulin
5. lysozyme
6. cytochrome C
7. glycine tetramer
Columns:

Mobile phase:
Flow rate:
Detection:
Temperature:
Samples:
TSKgel SuperSW columns,
4.6 mm ID × 30 cm
0.05% sodium azide aqueous solution
0.35 mL/min
RI
25 °C
polyethylene oxides (PEO) standards polyethylene glycols (PEG) standards,
(5 µL)

Tables within the tabs below summarize the molar mass ranges for the TSKgel SuperSW2000 and SuperSW3000 columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the particle pores.

 


Base Material:  Silica   
Particle Size (mean): 4 µm
 
Pore Size (mean): 12.5 nm  
Functional Group: Diol  
pH Stability: 2.5 - 7.5             Visit the TSKgel SuperSW2000 product page.
Calibration Range
(globular proteins):
5,000 - 150,000              
Calibration Range
(dextrans):
1,000 -
3 × 104 
 
Calibration Range
(polyethylene glycols 
and oxides):
500 - 15,000  


   

Base Material:  Silica   
Particle Size (mean): 4 µm
 
Pore Size (mean): 25 nm  
Functional Group: Diol  
pH Stability: 2.5 - 7.5             Visit the TSKgel SuperSW3000 product page.
Calibration Range 
(globular proteins):
1 × 104 -
5 × 105
            
Calibration Range 
(dextrans):
2,000 - 7 × 104  
Calibration Range 
(polyethylene glycols 
and oxides):
1,000 -
3.5 × 104