Solutions

TSKgel SW Column

TSKgel SW Columns for Protein Analysis

TSKgel SW columns, introduced in 1977, have become synonymous with analyzing protein molar masses in the field of biotechnology.

TSKgel SW columns are based on highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SW columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes.

The calibration curves are shown for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SW columns.


SW_Cal-curves.png

Column:
Mobile phase:

Flow rate:
Detection:
Samples:
TSKgel SW columns, 7.5 mm ID × 60 cm × 2
dextrans and polyethylene oxides: distilled water;
proteins: 0.3 mol/L NaCl in 0.1 mol/L phosphate buffer, pH 7.0
1.0 mL/min
UV @ 220 nm and RI
proteins, polyethylene oxides, dextrans

 

Tables within the tabs below summarize the molar mass ranges for the TSKgel G2000SW, G3000SW and G4000SW columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the particle pores. 

Base Material: Silica  
Particle Size (mean): 10 µm and 13 µm  
Pore size (mean): 12.5 nm  
Functional Group: Diol  
pH Stability: 2.5 - 7.5             Visit the TSKgel G2000SW product page.
Calibration Range
(globular proteins):
5,000 - 100,000 Da
 
Calibration Range
(dextrans):
1,000 - 3 × 104   
Calibration Range
(polyethylene glycols
and oxides):
500 - 15,000
 


   

Base Material:  Silica  
Particle Size (mean): 10 µm and 13 µm  
Pore Size (mean): 25 nm  
Functional Group: Diol                                       
pH Stability: 2.5 - 7.5               Visit the TSKgel G3000SW product page.
Calibration Range 
(globular proteins):
10,000 - 500,000 
 
Calibration Range
(dextrans):
2,000 - 7 × 104  
Calibration Range 
(polyethylene glycols
and oxides)
1,000 - 3.5 ×104  


   

Base Material:  Silica   
Particle Size (mean): 13 µm and 17 µm  
Pore Size (mean): 45 nm  
Functional Group: Diol  
pH Stability: 2.5 - 7.5             Visit the TSKgel G4000SW product page.
Calibration Range
(globular proteins):
20,000 - 7 × 106   
Calibration Range
(dextrans):
4,000 - 5 × 105   
Calibration Range
(polyethylene glycols
and oxides):
2,000 - 2.5 × 105