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TSKgel SW mAb Columns

 

TSKgel SW mAb Columns

The TSKgel SW mAb products consist of three specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAb can be analyzed using many different modes of HPLC, size exclusion is best for aggregation, dimer, and fragmentation, making it the best method for heterogeneity studies. The TSKgel SW mAb columns are:

  • TSKgel SuperSW mAb HR for high resolution analysis of mAb fragments, monomer and dimer

  • TSKgel SuperSW mAb HTP for high throughput analysis of mAb monomer and dimer with reduced internal diameter and length for fast run times (UHPLC compatible)

  • TSKgel UltraSW Aggregate for high resolution analysis of higher order mAb aggregates

The best performance of TSKgel SW mAb columns can be achieved when using them in chromatography systems with  minimized dwell volumes, such as modern UHPLC systems.

The TSKgel SW mAb columns utilize a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody. As shown in the figure, the calibration curve for the TSKgel SuperSW mAb HR column is similar to that of the TSKgel G3000SWXL column curve and has a shallower slope than the TSKgel UltraSW Aggregate column around the molecular weight range of gamma-globulin. This shallow calibration curve produces high resolution separations. The TSKgel UltraSW Aggregrate calibration curve shows a separation range up to around 2 million Da, which implies better resolution of aggregate/multimer of a mAb.

The TSKgel SW mAb HTP calibration curve is similar to the TSKgel SW mAb HR curve, since both columns are packed with the same resin and only differ in column dimensions.

SW_mAb_Cal-curves.png


Base Material: Silica  
Particle Size (mean): 4 µm  
Pore size (mean): 25 nm                           Visit the TSKgel SuperSW mAb HR product page.
Functional Group: Diol  
pH Stability: 2.5 - 7.5             
Calibration Range
(globular proteins):
10,000 - 500,000 Da
 


   

Base Material: Silica  
Particle Size (mean): 4 µm  
Pore size (mean): 25 nm                           Visit the TSKgel SuperSW mAb HTP product page.
Functional Group: Diol  
pH Stability: 2.5 - 7.5             
Calibration Range
(globular proteins):
10,000 - 500,000 Da
 


Base Material: Silica  
Particle Size (mean): 3 µm  
Pore size (mean): 30 nm                    Visit the TSKgel UltraSW Aggregate product page.
Functional Group: Diol  
pH Stability: 2.5 - 7.5             
Calibration Range
(globular proteins):
10,000 - 2,000,000 Da