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SWxl intro

TSKgel SWXL Columns for Protein Analysis

TSKgel SWXL columns, introduced in 1987, have become synonymous with analyzing protein molar masses in the field of biotechnology.

Particle Characteristics

TSKgel SWXL columns contain smaller particles than TSKgel SW columns; 5 and 8 μm versus 10 and 13 μm. As the TSKgel SW columns, TSKgel SWXL columns feature highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SWXL columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes. These columns are commonly used in the quality control of monoclonal antibodies and other biopharmaceutical products.

Calibration Curves

The figure shows the calibration curves for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SWXL columns. 

SWXL_Cal-curves.png  
Columns:

Mobile phase:

Detection:
Samples:
TSKgel SWXL columns, 
7.8 mm ID × 30 cm
0.3 mol/L NaCl in 0.1 mol/L sodium phosphate buffer, pH 7.0
UV @ 220 nm
1. thyroglobulin (6.6 × 105 Da)
2. IgG (1.56 × 105 Da)
3. bovine serum albumin
    (6.7 × 104 Da)
4. ovalbumin (4.3 × 104 Da)
5. peroxidase (4.02 × 104 Da)
6. β-lactoglobulin (3.5 × 104 Da)
7. myoglobin (1.69 × 104 Da)
8. ribonuclease A (1.37 × 104 Da)
9. cytochrome C (1.24 × 104 Da)
10. glycine tetramer (246 Da)

Tables within the tabs below summarize the molar mass ranges for the TSKgel G2000SWXL, G3000SWXL and G4000SWXL columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the pores.

 


Base Material: Silica   
Particle Size (mean): 5 µm
 
Pore Size (mean): 12.5 nm  
Functional Group: Diol  
pH Stability: 2.5 - 7.5               Visit the TSKgel G2000SWXL product page.
Calibration Range
(globular proteins):
5,000-150,000  
Calibration Range 
(dextrans):
1,000 -
3 × 104
 
Calibration Range
(polyethylene glycols
and oxides):
500 - 15,000  


   

Base Material:  Silica  
Particle Size (mean): 5 μm  
Pore Size (mean): 25 nm  
Functional Group Diol  
pH Stability 2.5 - 7.5                Visit the TSKgel G3000SWXL product page. 
Calibration Range
(globular proteins):
1 × 104 - 5 × 105   
Calibration Range
(dextrans):
2,000 - 7 × 104  
Calibration Range
(polyethylene glycols
and oxides):
1,000 - 3.5 × 104  


   

Base Material: Silica  
Particle Size (mean): 8 µm  
Pore Size (mean): 45 nm  
Functional Group: Diol  
pH Stability: 2.5 - 7.5            Visit the TSKgel G4000SWXL product page. 
Calibration Range
(globular proteins):
20,000 -
7 × 106 
 
Calibration Range
(dextrans):
4,000 - 5 × 105  
Calibration Range
(polyethylene glycols
and oxides): 
2,000 -
2.5 × 105