TSKgel® Alpha Columns for the Analysis of Polar Organic-Soluble Polymers
The main application area for TSKgel® Alpha columns is the analysis of polymers that are soluble in polar organic solvents. Examples of applications include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in DMF with 10 mmol/L LiBr), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in HFIP. These columns contain highly crosslinked hydroxylated polymethacrylate particles which minimize swelling in polar organic solvents.
Mixed bed TSKgel® Alpha Column
The mixed bed column, TSKgel® Alpha-M, has an extended linear calibration range, and is suitable for samples with a broad molar mass (MM) distribution as well as samples with unknown molecular mass.
The following figure shows the calibration curves of all Alpha columns.
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 |
Column:
Mobile phase:
Flow rate:
Detection:
Samples: |
TSKgel® Alpha columns, 6.0 mm ID × 15 cm
A. H2O
B. MeOH containing 10 mmol/L LiBr
C. DMF containing 10 mmol/L LiBr
0.6 mL/min
RI
A, B: polyethylene glycol (PEG) and polyethylene oxide (PEO) standards
C. polystyrene (PS) standards |
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Unlike TSKgel® PW-type columns, which are stable at most to a 50% organic mixed with water, TSKgel® Alpha columns offer excellent stability in 100% aqueous to 100% organic solvents. Suitable organic solvents are shown in the figure below.
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| Conditions of solvent change |
Flow rate:
Temperature:
Time for purge: |
1.0 mL/min
25 °C
8 h |
Conditions for TP measurement |
Flow rate:
Detection:
Temperature:
Sample: |
1.0 mL/min
RI
25 °C
ethylene glycol |
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