Solutions

TSKgel Alpha Columns

TSKgel Alpha Columns for the Analysis of Polar Organic-Soluble Polymers

The main application area for TSKgel Alpha columns is the analysis of polymers that are soluble in polar organic solvents. Examples of applications include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in DMF with 10 mmol/L LiBr), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in HFIP. These columns contain highly crosslinked hydroxylated polymethacrylate particles which minimize swelling in polar organic solvents.

Mixed bed TSKgel Alpha Column

The mixed bed column, TSKgel Alpha-M, has an extended linear calibration range, and is suitable for samples with a broad molar mass (MM) distribution as well as samples with unknown molecular mass.

The following figure shows the calibration curves of all Alpha columns.

Alpha_Cal-curves.png

Column:
Mobile phase:


Flow rate:
Detection:
Samples:
TSKgel SuperAW columns, 6.0 mm ID × 15 cm
A. H2O
B. MeOH containing 10 mmol/L LiBr
C. DMF containing 10 mmol/L LiBr
0.6 mL/min
RI
A, B: polyethylene glycol (PEG) and polyethylene oxide (PEO) standards
C. polystyrene (PS) standards

Unlike TSKgel PW-type columns, which are stable at most to a 50% organic mixed with water, TSKgel Alpha columns offer excellent stability in 100% aqueous to 100% organic solvents. Suitable organic solvents are shown in the figure below.

Alpha-3000_Solvent-compatibility.png
Conditions of solvent change
Flow rate:
Temperature:
Time for purge:
1.0 mL/min
25 °C
8 h

Conditions for TP measurement
Flow rate:
Detection:
Temperature:
Sample:
1.0 mL/min
RI
25 °C
ethylene glycol


Base Material: hydrophilic methacylate  
Particle Size (mean): 7 µm  
Pore size (mean): 2.5 nm  
Exclusion Limit 
(Polyethylene oxide):
5,000 Da (water)                   Visit the TSKgel Alpha-2500 product page.
Exclusion Limit
(Polystyrene - THF):
10,000 Da
Shipping Solvent: water
Max. Pressure Drop: 4 MPa  


   

Base Material: hydrophilic methacylate  
Particle Size (mean): 7 µm  
Pore size (mean): 15 nm  
Exclusion Limit 
(Polyethylene oxide):
90,000 Da (water)                   Visit the TSKgel Alpha-3000 product page.
Exclusion Limit
(Polystyrene - THF):
100,000 Da
Shipping Solvent: water
Max. Pressure Drop: 4 MPa  


   

Base Material: hydrophilic methacylate  
Particle Size (mean): 10 µm   
Pore size (mean): 45 nm   
Exclusion Limit 
(Polyethylene oxide):
40,000 Da (water)                   Visit the TSKgel Alpha-4000 product page.
Exclusion Limit
(Polystyrene - THF):
1 million Da
Shipping Solvent: water
Max. Pressure Drop: 3 MPa  


   

Base Material: hydrophilic methacylate  
Particle Size (mean): 10 µm  
Pore size (mean): 100 nm  
Exclusion Limit 
(Polyethylene oxide):
1 million Da (water)                   Visit the TSKgel Alpha-5000 product page.
Exclusion Limit
(Polystyrene - THF):
7 million Da
Shipping Solvent: water
Max. Pressure Drop: 3 MPa  


   

Base Material: hydrophilic methacylate  
Particle Size (mean): 13 µm  
Pore size (mean): ND   
Exclusion Limit 
(Polyethylene oxide):
> 1 × 107 Da (water)                   Visit the TSKgel Alpha-6000 product page.
Exclusion Limit
(Polystyrene - THF):
> 10 million Da
Shipping Solvent: water
Max. Pressure Drop: 2 MPa  


   

Base Material: hydrophilic methacylate  
Particle Size (mean): 13 µm  
Exclusion Limit 
(Polyethylene oxide):
> 107 Da (water)                   Visit the TSKgel Alpha-M product page.
Exclusion Limit
(Polystyrene - THF):
> 10 million Da
Shipping Solvent: water
Max. Pressure Drop: 2 MPa