Solutions

TSKgel SuperHZ Columns

TSKgel SuperHZ Columns for Reduced Polyer Adsorption and Limited Solvent Range

The TSKgel SuperHZ column line consists of five columns with different pore sizes, TSKgel SuperHZ1000 through TSKgel SuperHZ4000, and three columns with an extended linear range of the calibration curve, TSKgel SuperHZM-N, SuperHZM-M and SuperHZM-H. The calibration curves are shown below.

Main characteristics:
  • TSKgel SuperHZ columns have been developed for high throughput, high efficiency GPC applications such as those encountered in combinatorial chemistry experiments.
  • These columns are based on the TSKgel HXL column line, differing only in dimensions (4.6 and 6.0 mm ID x 15 cm) and particle size. The benefits of TSKgel SuperHZ columns over conventional TSKgel HXL columns are shown in the following chromatogram.
  • Ultra-low sample adsorption, i.e., the columns show true size exclusion behavior for most polymers
  • Maximum operating temperatures for TSKgel SuperHZ-type columns are 60 °C for TSKgel SuperHZ1000, SuperHZ2000, SuperHZ2500 and SuperHZ3000, and 80 °C for the remaining columns in the product line.
  • Limited solvent range. The columns are shipped in THF, which can be exchanged for a limited number of organic solvents as shown below.

Beware of extra-column bandbroadening

Column dimensions are 6 mm ID x 15 cm and 4.6 mm ID x 15 cm versus 7.8 mm ID x 30 cm for conventional GPC columns. The smaller column dimensions translate to a reduction of peak volume by a factor of 3.4 (6 mm ID) and a factor of 5.8 (4.6 mm ID) versus the same component eluting from a corresponding TSKgel HXL column. Thus, your HPLC system may require optimization of components that can give rise to extra-column band broadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.

Columns with linear calibration curves

The TSKgel SuperHZ product line contains three mixed bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The mixed bed columns feature increasing linear calibration ranges, from TSKgel SuperHZM-L, SuperHZM-N,  to SuperHZM-H.

The calibration curves for each of the TSKgel SuperHZ columns are shown below.

 


Solvent compatibility for TSKgel SuperHZ columns
 
The column shipping solvent limits the types of solvents that can be used with each column type.

 

Recommended Solvent

Application

THF polystyrene, epoxy resin, phenoxy resin, polycarbonate, 
polyisoprene, polyvinyl acetate, polyvinyl chloride, 
monoglycerides, fatty acids, poly(butadiene), 
polybutadiene, poly(methyl methacrylate), 
poly(styrene-butadiene), poly(styrene-acrylonitrile)
N,N-Dimethylformamide (DMF) +/- 5mM LiBr
polyvinyl chloride, polyvinyl fluoride, urea resins, 
polyurethane, polystyrene, polyester, 
polyimido ether, polyimido ester, 
polyphenol (aqueous solution), polyacrylonitrile
o-Dichlorobenzene (ODCB)
polyethylene, polypropylene
Chloroform polycarboxylic ether, acrylic resin, 
epoxy resin, polystyrene
m-Cresol/Chloroform nylon, polyester, polyamide, 
poly(ethylene terephthalate)
Toluene
polybutadiene, polysiloxane

 

TSKgel SuperHZ-type columns are packed (and shipped) in tetrahydrofuran. The table below lists the solvents that may be used to replace the original shipping solvent.

Solvent 

Can Be Replaced With

Tetrahydrofuran 2,3 
benzene, chloroform, toluene, xylene, 
dichloromethane, dichloroethane
Acetone
carbon tetrachloride, o-dichlorobenzene, dimethylformamide, 
dodecane, dimethyl sulfoxide, dioxane, ethylacetate, 
FC-113, hexane, pyridine, hexafluoroisopropanol/chloroform, 
methyl ethyl ketone, quinoline, cyclohexane 
Chloroform
m-cresol in chloroform, up to 10%
hexafluoroisopropanol in chloroform
Dimethylformamide
dimethyl sulfoxide, dioxane, tetrahydrofuran, toluene 
o-Dichlorobenzene
1-chloronaphthalene, trichlorobenzene

Notes:

  1. Keep flow rate below <0.5mL/min during solvent change. Solvent can be changed one way/one time only.
  2. All TSKgel SuperHZ analytical columns are shipped containing tetrahydrofuran (THF).
  3. Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.

* 100% methanol cannot be used with TSKgel H-type columns; use this solvent with TSKgel SW-type or Alpha columns.

 

1-chloronaphthalene, trichlorobenzene
1-chloronaphthalene, trichlorobenzene
1-chloronaphthalene, trichlorobenzene
1-chloronaphthalene, trichlorobenzene

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 3 µm  
Pore size (mean): 1.5 nm  
Exclusion Limit: 1,000 Da             Visit the TSKgel SuperHZ1000 product page.
Max. Temp. (ºC): 60           
Shipping Solvent: THF
 

   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 3 µm  
Pore size (mean): 2.0 nm  
Exclusion Limit: 10,000 Da             Visit the TSKgel SuperHZ2000 product page.
Max. Temp. (ºC): 60           
Shipping Solvent: THF


   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 3 µm  
Pore size (mean): 3.0 nm  
Exclusion Limit: 20,000 Da             Visit the TSKgel SuperHZ2500 product page.
Max. Temp. (ºC): 60           
Shipping Solvent: THF


   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 3 µm  
Pore size (mean): 7.5 nm  
Exclusion Limit: 60,000 Da             Visit the TSKgel SuperHZ3000 product page.
Max. Temp. (ºC): 60           
Shipping Solvent: THF


   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 3 µm  
Pore size (mean): 2.0 nm  
Exclusion Limit: 4 x 105 Da             Visit the TSKgel SuperHZ4000 product page.
Max. Temp. (ºC): 80           
Shipping Solvent: THF


   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 10 µm  
Pore size (mean): mixed   
Exclusion Limit: 400 x 106 Da             Visit the TSKgel SuperHZM-H product page.
Max. Temp. (ºC): 80           
Shipping Solvent: THF


   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 3 µm  
Pore size (mean): mixed  
Exclusion Limit: 4 x 106 Da             Visit the TSKgel SuperHZM-M product page.
Max. Temp. (ºC): 80           
Shipping Solvent: THF


   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 3 µm  
Pore size (mean): mixed  
Exclusion Limit: 700,000 Da             Visit the TSKgel SuperHZM-N product page.
Max. Temp. (ºC): 80           
Shipping Solvent: THF