TSKgel® SuperHZ Columns

TSKgel^® SuperHZ Columns for Reduced Polyer Adsorption and Limited Solvent Range

The TSKgel^® SuperHZ column line consists of five columns with different pore sizes, TSKgel^® SuperHZ1000 through TSKgel^® SuperHZ4000, and three columns with an extended linear range of the calibration curve, TSKgel^® SuperHZM-N, SuperHZM-M and SuperHZM-H. The calibration curves are shown below.

Main characteristics:

• TSKgel^® SuperHZ columns have been developed for high throughput, high efficiency GPC applications such as those encountered in combinatorial chemistry experiments.
• These columns are based on the TSKgel^® HXL column line, differing only in dimensions (4.6 and 6.0 mm ID x 15 cm) and particle size. The benefits of TSKgel^® SuperHZ columns over conventional TSKgel^® HXL columns are shown in the following chromatogram.
• Ultra-low sample adsorption, i.e., the columns show true size exclusion behavior for most polymers
• Maximum operating temperatures for TSKgel^® SuperHZ-type columns are 60 °C for TSKgel^® SuperHZ1000, SuperHZ2000, SuperHZ2500 and SuperHZ3000, and 80 °C for the remaining columns in the product line.
• Limited solvent range. The columns are shipped in THF, which can be exchanged for a limited number of organic solvents as shown below.

Beware of extra-column bandbroadening

Column dimensions are 6 mm ID x 15 cm and 4.6 mm ID x 15 cm versus 7.8 mm ID x 30 cm for conventional GPC columns. The smaller column dimensions translate to a reduction of peak volume by a factor of 3.4 (6 mm ID) and a factor of 5.8 (4.6 mm ID) versus the same component eluting from a corresponding TSKgel HXL column. Thus, your HPLC system may require optimization of components that can give rise to extra-column band broadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.

Columns with linear calibration curves

The TSKgel^® SuperHZ product line contains three mixed bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The mixed bed columns feature increasing linear calibration ranges, from TSKgel^® SuperHZM-L, SuperHZM-N,  to SuperHZM-H.

The calibration curves for each of the TSKgel^® SuperHZ columns are shown below.

Solvent compatibility for TSKgel^® SuperHZ columns 

TSKgel^® SuperHZ-type columns are packed (and shipped) in tetrahydrofuran. The table below lists the solvents that may be used to replace the original shipping solvent.

Notes:

1. Keep flow rate below <0.5mL/min during solvent change. Solvent can be changed one way/one time only.
2. All TSKgel^® SuperHZ analytical columns are shipped containing tetrahydrofuran (THF).
3. THF in TSKgel^® SuperHZ1000 columns cannot be replaced with dichloromethane or dichloroethane.
4. Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.

* 100% methanol cannot be used with TSKgel^® H-type columns; use this solvent with TSKgel^® SW-type or Alpha columns.