Products

HPLC Columns

TSKgel UP-SW3000

TSKgel UP-SW3000 columns are designed for the analysis of monoclonal antibodies and other biopharma products and can be used on both HPLC and UHPLC systems. The columns are packed with 2 μm silica-based beads shielded with a hydrophilic diol-type bonded phase that prevents the silica surface from interacting with protein samples.  

 

TSKgel UP-SW3000 columns feature the same pore size as the well-established TSKgel G3000SWxl columns. Hence methods developed using TSKgel G3000SWxl columns can easily be transferred to TSKgel UP-SW3000 columns on conventional HPLC systems as well as on UHPLC systems.

 

TSKgel UP-SW3000 columns offer higher resolution, improved peak shape and increased efficiency yielding methods that are robust, reproducible and easily transferrable between UHPLC and HPLC systems.

 

TSKgel UP-SW3000 columns offer superior reproducibility injection-to-injection, from column-to-column within the same lot and from lot-to-lot. %RSD values for peak parameters including retention times, area peak height, peak asymmetry, and theoretical plate count demonstrate the exceptional reproducibility of these columns.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
23449TSKgel UP-SW30002Stainless Steel4.6151,554
23448TSKgel UP-SW30002Stainless Steel4.6302,095
          

Product Attributes

Base material: silica
Particle size (mean): 2 µm
Pore size (mean): 25 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range (proteins): 10 - 500 kDa

Protein Calibration Curves

SEC_UP-SW3000_AN95--fig-1.png

Column: TSKgel UP-SW3000, 2 µm,
4.6 mm ID × 15 cm
Mobile phase: 100 mmol/L phosphate buffer, pH 6.7, + 100 mmol/L Na2SO4 + 0.05% NaN3
Flow rate: 0.35 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 5 µL
Samples: 1' Thyroglobulin dimer
1. Thyroglobulin, 640,000 Da
2' γ-globulin dimer
2. γ-globulin 155,000 Da
3. Ovalbumin, 47,000 Da
4. Riboneclease A, 13,000 Da
5. p-aminobenzoic acid, 137 Da

                    

mAb Aggregate Analysis

 

SEC_UP-SW3000_AN95--fig-2_rev2.png

 

Columns: TSKgel UP-SW3000, 2 µm,
4.6 mm ID × 15 cm
Mobile phase: 100 mmol/L sodium phosphate buffer, pH 6.8 + 100 mmol/L sodium sulfate + 0.05% sodium azide
Gradient: isocratic (0-7 min)
Flow rate: 0.35 mL/min
LC system: Ultimate® 3000RS UHPLC system
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 10 µL
Sample: mAb (0.4 mg/mL)

 

Fast Analysis of mAb Sample

 

SEC_UP-SW3000_AN95--fig-3_rev2.png

 

Columns: TSKgel UP-SW3000, 2 µm,
4.6 mm ID × 15 cm
Mobile phase: 100 mmol/L sodium phosphate buffer, pH 6.8 + 100 mmol/L sodium sulfate + 0.05% sodium azide
Gradient: isocratic (0-4 min)
Flow rate: 0.5 mL/min
LC system: Ultimate® 3000RS UHPLC system
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 10 µL
Sample: mAb (0.4 mg/mL)

 


Comparison of QC Standard Protein Mixture

 

SEC_UP-SW3000_AN89--fig-1A_rev.png

 

Columns: TSKgel UP-SW3000, 2 µm,
4.6 mm ID × 15 cm
Competitor column, 1.7 µm, 4.6 mm ID × 15 cm
Mobile phase: 100 mmol/L sodium phosphate buffer, pH 6.7 + 100 mmol/L Na2SO4 + 0.05% NaN3
Gradient: isocratic 
Flow rate: 0.35 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 5 µL
Sample: QC standard protein test mixture:
thyroglobulin, 640 kDa, 0.5 g/L
γ-globulin, 155 kDa, 1 g/L
ovalbumin, 47 kDa, 1 g/L
ribonuclease A, 13,700 Da, 1.5 g/L
p-aminobenzoic acid, 137 Da, 0.01 g/L

 

Comparison of mAb Analysis

 

SEC_UP-SW3000_AN89_Fig2_rev.png

 

Columns: TSKgel UP-SW3000, 2 µm,
4.6 mm ID × 30 cm
Competitor column, 1.7 µm, 4.6 mm ID × 15 cm
Mobile phase: 100 mmol/L sodium phosphate buffer, pH 6.7 + 100 mmol/L Na2SO4 + 0.05% NaN3
Gradient: isocratic
Flow rate: 0.35 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Sample: mAb 

 

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AN88 Increased Monoclonal Antibody Resolution with TSKgel UP-SW3000 Columns Application Note
AN89 Increased Resolution and Reproducibility with TSKgel UP-SW3000 Columns Versus a Competitive SEC Column Application Note
AN90 Improving Resolution and Sample Throughput for Protein Analysis using TSKgel UP-SW3000 Columns Application Note
AN95 Rapid and accurate therapeutic mAb aggregate analysis using a TSKgel UP-SW3000, 2μm, SEC Column Application Note
AN97 The Usage of Isopropyl Alcohol in Size Exclusion Chromatography for Monoclonal Antibody Separation Application Note
DS1241 TSKgel UP-SW3000 ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
PD17 Superior Reproducibility of TSKgel UP-SW3000 Columns Performance Data
PO43 TSKgel UP-SW3000, 2 μm UHPLC/HPLC SEC Columns Product Overview
TP214 Characterization of New 2 μm Particle Size, 25 nm Pore Size Analytical Size Exclusion Chromatography Column With Larger Exclusion Limit Technical Presentation
TP215 Mass Spectrometric Characterization of Intact Half-mAb separated from intact monoclonal antibody and fragments using analytical size exclusion chromatography column Technical Presentation
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
23451TSKgel UP-SW3000 DC Guard Column for 4.6 mm ID column2Stainless Steel4.62609
23450TSKgel UP-SW3000 Guard Column for 4.6 mm ID column2Stainless Steel4.62609