Service & Support

Process Media - Affinity FAQ's

 

The following are Affinity Process Media FAQ's. Please click on the corresponding tab below for the questions and answers.

  1. Where do I find the coupling procedure for the activated resins?

  2. Can you clean affinity resins with caustic?

  3. Does Tosoh Bioscience offer a Protein A resin for antibody purification?


 

1. Where do I find the coupling procedure for the activated resins?

Contact the Technical Support line at 800-366-4875 and a technical specialist can provide a detailed procedure for the activated resins. The Tosoh Bioscience Literature Library also has many recommended prodcedures based on the type of reactive or activated resin being used.

 

2. Can you clean affinity resins with caustic?

TOYOPEARL AF-Chelate-650M can be cleaned with caustic up to 0.5 mol/L NaOH. All other Affinity products such as the activated media are most likely sensitive to caustic (due to the nature of the attached ligand) and should be cleaned with less aggressive combinations of salt, chaotrope, and detergent.

 

3. Does Tosoh Bioscience offer a Protein A resin for antibody purification?

Yes, Tosoh Bioscience offers two Protein A resins: TOYOPEARL AF-rProtein A-650F and our most recently introduced high capacity resin, TOYOPEARL AF-rProtein A HC-650F.

TOYOPEARL AF-rProtein A HC-650F resin exhibits dynamic binding capacities of greater than 65 g/L at residence times of 5 minutes and greater than 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L.

TOYOPEARL AF-rProtein A-650F offers alkaline resistance and low ligand leakages in the range 5-25 ng/mg IgG.