Solutions

TSKgel PW

TSKgel PW Columns for Analysis of Water-Soluble Polymers

TSKgel PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12 µm for the smaller pore size columns to 17 µm for the larger pore size columns.

The main application areas for TSKgel PW columns are the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides.

The figure below shows the calibration curves for all TSKgel PW columns. Suitable solvents for various polymer samples are shown in the table below.

PW_Cal-curves.png

Columns:


                         


Mobile phase:
Flow rate:
Detection:
TSKgel PW columns,
all 7.5 mm ID × 60 cm
A. G2000PW, B. G2500PW,
C. G3000PW, D. G4000PW,
E. G5000PW, F. G6000PW,
G. GMPW
distilled H20
1.0 mL/min
RI

Specialty TSKgel PW Columns

The TSKgel GMPW column is a mixed bed column containing a mixture of different pore sizes that has an extended linear calibration range, suitable for samples with a broad molar mass (MM) distribution, as well as unknown samples. A TSKgel G6000PW column is available in PEEK hardware, TSKgel BioAssist G6PW, when ultra-low sample adsorption is required, such as in virus analysis.

Recommended eluents for GFC of water-soluble polymers on TSKgel PW columns

Type of Polymer 

Typical Sample

Suitable Eluent

Nonionic hydrophilic

polyyethylene glycol

soluble starch, methyl cellulose, 

pullulan dextran, hydroxyethyl cellulose 

polyvinyl alcohol, polyacrylamide

 

distilled water
0.01 mol/L NaOH
20% DMSO
Buffer or salt solution (e.g. 0.1-0.5 mol/L NaNO3)

 

Nonionic Hydrophobic

 

polyvinylpyrrolidone

Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3)

Anionic Hydrophilic

sodium chondroitin sulfate, sodium alginate, carboymethyl cellulose, sodium polyacrylate, sodium hyaluronate

Buffer or salt solution (e.g. 0.1 mol/L NaNO3)

Anionic Hydrophobic

sulfonated lignin sodium salt,
sodium polystyrenesulfonate

Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3)

Cationic Hydrophilic

glycol chitosan, DEAE-dextran, poly(ethyleneimine), poly(trimethylaminoethyl methacrylate) iodide salt

0.5 mol/L acetic acid with 0.3 mol/L Na2SO4 or 0.8 mol/L NaNO3

Cationic Hydrophobic

poly(4-vinylbenzyltrimethylammonium chloride), poly(N-methyl-2-vinylpyridinium) iodide salt

0.5 mol/L acetic acid with 0.3 mol/L Na2SO4

Amphoteric Hydrophilic

peptides, proteins, poly- and oligosaccharides, DNA, RNA

Buffer or salt solution (e.g. 0.1 mol/L NaNO3)

Amphoteric hydrophobic
 

blue dextran, collagen, gelatin, hydrophobic proteins, hydrophobic peptides

Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3 or 35-45% CH3CN in 0.1% TFA)


Base Material: hydroxylated polymethacylate  
Particle Size (mean): 12 µm  
Pore size (mean): 12.5 nm  
Calibration Range
(polyethylene glycols
and oxides):
up to 2,000 Da             Visit the TSKgel G2000PW product page.
Max. % Organic: 20%           
Shipping Solvent: water  


   

Base Material: hydroxylated polymethacrylate  
Particle Size (mean): 12 µm and 17 µm  
Pore size (mean): <20 nm  
Calibration Range
(polyethylene glycols
and oxides):
up to 3,000 Da             Visit the TSKgel G2500PW product page.
Max. % Organic: 20%             
Shipping Solvent: water
 




Base Material: hydroxylated polymethacylate  
Particle Size (mean): 12 µm and 17 µm
 
Pore size (mean): 20 nm  
Calibration Range 
(polyethylene glycols 
and oxides):
up to 50,000 Da             Visit the TSKgel G3000PW product page.
Max. % Organic: 20%           
Shipping Solvent: water  


   

Base Material: hydroxylated polymethacylate  
Particle Size (mean): 17 µm  
Pore size (mean): 50 nm  
Calibration Range 
(polyethylene glycols 
and oxides):
up to 300,000 Da             Visit the TSKgel G4000PW product page.
Max. % Organic: 20%           
Shipping Solvent: water  


   

Base Material: hydroxylated polymethacylate  
Particle Size (mean): 17 µm  
Pore size (mean): 100 nm  
Calibration Range 
(polyethylene glycols 
and oxides):
up to 1,000,000 Da             Visit the TSKgel G5000PW product page.
Max. % Organic: 20%           
Shipping Solvent: water  


   

Base Material: hydroxylated polymethacylate  
Particle Size (mean): 17 µm  
Pore size (mean): >100 nm  
Calibration Range 
(polyethylene glycols 
and oxides):
up to 8,000,000 Da             Visit the TSKgel G6000PW product page.
Max. % Organic: 20%           
Shipping Solvent: water  


   

Base Material: hydroxylated polymethacylate  
Particle Size (mean): 17 µm  
Pore size (mean): <10 - 100 nm  
Calibration Range 
(polyethylene glycols 
and oxides):
500 - 8,000,000 Da             Visit the TSKgel GMPW product page.
Max. % Organic: 20%           
Shipping Solvent: water